Journal: Cell reports
Article Title: Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A
doi: 10.1016/j.celrep.2022.110309
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: BMDMs were transfected with empty vector control (pcDNA3.1-(empty)-TAG, Addgene 138209), constitutively active STAT3 (Stat3-C Flag pRc/CMV, Addgene 8722), or constitutively active IKK-2 (IKK-2 S177E S181E, Addgene 11105) using a mouse macrophage transfection system, according to the manufacturer’s instructions (Lonza, VPA-1009; Mouse Macrophage Nucleofector Kit).
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Transcription Factor Assay, Chromatin Immunoprecipitation, Transgenic Assay, Software